Cells: A Laboratory Manual - Tapa dura

Having identified a gene product, how do you determine what it does? This three-volume study seeks to answer this question, and provides strategies and techniques for the study of cellular components. It contains step-by-step protocols of proven reliability, ranging widely from basic culture of vertebrate and non-vertebrate cells, metabolic labelling, purification of organelles, introduction and visualization of molecules in live cells, through advanced detection methods using cytochemical, immunological and in situ hybridization approaches, and imaging techniques such as fluorescence, confocal and electron microscopy.